Bosch 480 Series Návod k obsluze stáhnout pdf (Strana 16)

LightCycler® 480 Gene Scanning Software

Gene Scanning with the LightCycler® 480 System

High Resolution Melting

This ﬁgure shows how a High Resolution Melting experiment can detect both homozy-

gous and heterozygous allelic variants in a sample. Homozygous variants are detectable

because their melting curves are displaced along the temperature axis (x-axis) relative to

homozygous “wild type” samples. Heterozygous variants have melting curves that differ

even more dramatically in shape from “wild type” curves.

In heterozygous samples, melting curve shape changes because the observed melting

curve is actually a composite of both heteroduplex and homoduplex components.

Heteroduplexes formed in the sample (i.e., between the “wild type” and variant

strands) are less stable than the homoduplexes formed, and thus, dissociate more

readily.

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Bosch 480 Series Návod k obsluze Strana 16